English champions Arsenal will face Italian Serie A runners-up Fiorentina in the last 32 of the Women’s Champions League.
The Gunners, who are unseeded, host the Italians in the first leg.
Seeded Manchester City, who were second in the Women’s Super League last term, were drawn against Swiss side Lugano.
Scottish champions Glasgow City face a trip to Russia to face Chertanovo, while qualifiers Hibernian will take on Czech side Slavia Prague.
It is the first time two Scottish sides have made the knockout stages, after Hibs beat Welsh side Cardiff Met in qualifying.
Lyon, who have won the last four titles and have England quartet Lucy Bronze, Nikita Parris, Alex Greenwood and Izzy Christiansen in their squad, will travel to Russian side Ryazan-VDV in the opening leg of their tie.
Italian champions Juventus, who have former England striker Eni Aluko, meet Spanish league runners-up Barcelona in arguably the tie of the round.
Ties will be played over two legs in September, with the first legs on 11-12 September and the second legs on 25-26 September.
Juventus v Barcelona
Hibernian v Slavia Prague
ZFK Spartak v Atletico Madrid
Braga v Paris Saint-Germain
Vllaznia v Fortuna Hjørring
Chertanovo v Glasgow City
Ryazan-VDV v Lyon
Arsenal v Fiorentina
IFK Gothenburg v Bayern Munich
St. Polten v FC Twente
Anderlecht v BIIK-Kazygurt
Breidablik v Sparta Prague
Mitrovica v Wolfsburg
Pitea v Brondby
Lugano v Man City
FC Minsk v FC Zurich